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"85_TVKRTF_11|u1", "860_TVKRTF_11|u1", "861_TVKRTF_11|u1", "862_TVKRTF_11|u1", "863_TVKRTF_11|u1", "864_TVKRTF_11|u1", "865_TVKRTF_11|u1", "866_TVKRTF_11|u1", "867_TVKRTF_11|u1", "868_TVKRTF_11|u1", "869_TVKRTF_11|u1", "86_TVKRTF_11|u1", "870_TVKRTF_11|u1", "871_TVKRTF_11|u1", "872_TVKRTF_11|u1", "873_TVKRTF_11|u1", "874_TVKRTF_11|u1", "875_TVKRTF_11|u1", "876_TVKRTF_11|u1", "878_TVKRTF_11|u1", "879_TVKRTF_11|u1", "87_TVKRTF_11|u1", "880_TVKRTF_11|u1", "881_TVKRTF_11|u1", "882_TVKRTF_11|u1", "883_TVKRTF_11|u1", "884_TVKRTF_11|u1", "885_TVKRTF_11|u1", "886_TVKRTF_11|u1", "886_TVKRTF_11|u2", "887_TVKRTF_11|u1", "888_TVKRTF_11|u1", "889_TVKRTF_11|u1", "88_TVKRTF_11|u1", "890_TVKRTF_11|u1", "891_TVKRTF_11|u1", "892_TVKRTF_11|u1", "893_TVKRTF_11|u1", "894_TVKRTF_11|u1", "895_TVKRTF_11|u1", "896_TVKRTF_11|u1", "897_TVKRTF_11|u1", "898_TVKRTF_11|u1", "899_TVKRTF_11|u1", "89_TVKRTF_11|u1", "8_TVKRTF_11|u1", "8_TVKRTF_11|u2", "900_TVKRTF_11|u1", "901_TVKRTF_11|u1", "902_TVKRTF_11|u1", "903_TVKRTF_11|u1", "904_TVKRTF_11|u1", "905_TVKRTF_11|u1", "906_TVKRTF_11|u1", "907_TVKRTF_11|u1", "908_TVKRTF_11|u1", "908_TVKRTF_11|u2", "909_TVKRTF_11|u1", "910_TVKRTF_11|u1", "911_TVKRTF_11|u1", "912_TVKRTF_11|u1", "913_TVKRTF_11|u1", "914_TVKRTF_11|u1", "915_TVKRTF_11|u1", "916_TVKRTF_11|u1", "917_TVKRTF_11|u1", "918_TVKRTF_11|u1", "919_TVKRTF_11|u1", "91_TVKRTF_11|u1", "920_TVKRTF_11|u1", "921_TVKRTF_11|u1", "922_TVKRTF_11|u1", "923_TVKRTF_11|u1", "924_TVKRTF_11|u1", "925_TVKRTF_11|u1", "926_TVKRTF_11|u1", "927_TVKRTF_11|u1", "928_TVKRTF_11|u1", "929_TVKRTF_11|u1", "92_TVKRTF_11|u1", "930_TVKRTF_11|u1", "931_TVKRTF_11|u1", "933_TVKRTF_11|u1", "934_TVKRTF_11|u1", "935_TVKRTF_11|u1", "936_TVKRTF_11|u1", "937_TVKRTF_11|u1", "939_TVKRTF_11|u1", "93_TVKRTF_11|u1", "940_TVKRTF_11|u1", "941_TVKRTF_11|u1", "942_TVKRTF_11|u1", "943_TVKRTF_11|u1", "944_TVKRTF_11|u1", "945_TVKRTF_11|u1", "946_TVKRTF_11|u1", "947_TVKRTF_11|u1", "948_TVKRTF_11|u1", "948_TVKRTF_11|u2", "949_TVKRTF_11|u1", "94_TVKRTF_11|u1", "950_TVKRTF_11|u1", "951_TVKRTF_11|u1", "952_TVKRTF_11|u1", "953_TVKRTF_11|u1", "954_TVKRTF_11|u1", "955_TVKRTF_11|u1", "956_TVKRTF_11|u1", "957_TVKRTF_11|u1", "958_TVKRTF_11|u1", "959_TVKRTF_11|u1", "95_TVKRTF_11|u1", "960_TVKRTF_11|u1", "961_TVKRTF_11|u1", "962_TVKRTF_11|u1", "963_TVKRTF_11|u1", "964_TVKRTF_11|u1", "965_TVKRTF_11|u1", "966_TVKRTF_11|u1", "967_TVKRTF_11|u1", "968_TVKRTF_11|u1", "969_TVKRTF_11|u1", "96_TVKRTF_11|u1", "970_TVKRTF_11|u1", "971_TVKRTF_11|u1", "972_TVKRTF_11|u1", "974_TVKRTF_11|u1", "975_TVKRTF_11|u1", "976_TVKRTF_11|u1", "977_TVKRTF_11|u1", "977_TVKRTF_11|u2", "978_TVKRTF_11|u1", "979_TVKRTF_11|u1", "97_TVKRTF_11|u1", "980_TVKRTF_11|u1", "981_TVKRTF_11|u1", "983_TVKRTF_11|u1", "984_TVKRTF_11|u1", "985_TVKRTF_11|u1", "985_TVKRTF_11|u2", "986_TVKRTF_11|u1", "987_TVKRTF_11|u1", "988_TVKRTF_11|u1", "989_TVKRTF_11|u1", "98_TVKRTF_11|u1", "990_TVKRTF_11|u1", "991_TVKRTF_11|u1", "993_TVKRTF_11|u1", "994_TVKRTF_11|u1", "995_TVKRTF_11|u1", "996_TVKRTF_11|u1", "997_TVKRTF_11|u1", "998_TVKRTF_11|u1", "999_TVKRTF_11|u1", "99_TVKRTF_11|u1", "9_TVKRTF_11|u1" ], "qualifying_reads": 0, "qualifying_fraction": 0.0, "qualifying_read_ids": [], "candidate_read_species": {}, "qualifying_read_loci": {}, "status": "NOT_DETECTED", "candidates": { "e_coli": { "label": "Escherichia coli", "role": "spikein_candidate", "reads": 0, "fraction": 0.0, "loci": 0, "status": "NOT_DETECTED", "fraction_ci95": [ 0, 0.0008082446793096467 ] }, "d_melanogaster": { "label": "Drosophila melanogaster", "role": "spikein_candidate", "reads": null, "fraction": null, "loci": null, "status": "HOST_NOT_DISTINGUISHABLE", "fraction_ci95": [ null, null ] }, "s_cerevisiae": { "label": "Saccharomyces cerevisiae", "role": "spikein_candidate", "reads": 0, "fraction": 0.0, "loci": 0, "status": "NOT_DETECTED", "fraction_ci95": [ 0, 0.0008082446793096467 ] }, "s_pombe": { "label": "Schizosaccharomyces pombe", "role": "spikein_candidate", "reads": 0, "fraction": 0.0, "loci": 0, "status": "NOT_DETECTED", "fraction_ci95": [ 0, 0.0008082446793096467 ] }, "lambda": { "label": "Lambda phage", "role": "sequencing_control", "reads": 0, "fraction": 0.0, "loci": 0, "status": "NOT_DETECTED", "fraction_ci95": [ 0, 0.0008082446793096467 ] }, "phix": { "label": "PhiX174", "role": "sequencing_control", "reads": 0, "fraction": 0.0, "loci": 0, "status": "NOT_DETECTED", "fraction_ci95": [ 0, 0.0008082446793096467 ] } }, "assignment_counts": { "ambiguous_or_unassigned": 3622, "host": 1127 }, "fraction_ci95": [ 0, 0.0008082446793096467 ], "zero_hit_upper95": 0.0006306143605705472, "command": [ "/path/to/bowtie2", "--very-sensitive-local", "-p", "4", "-x", "/path/to/index", "-U", "/path/to/HL120.inserts.fastq.gz" ], "database_manifest": "/path/to/fca8c364a09091ce629dc3cc008728063620b30003fc46098d56bbbbdbbe18a4.json", "parameters": { "mapq": 20, "minimum_aligned_bp": 80, "minimum_query_coverage": 0.8, "minimum_identity": 0.85, "minimum_score_margin": 10 }, "interpretation": "Candidate foreign-sequence signal, not proof that spike-in was intentionally added. No spike-in was declared for these libraries; positive signals require investigation. Zero hits do not establish absence, and the binomial bound assumes independent inserts and perfect detectability. Host and candidate genomes plus Mollicutes/bacterial decoys compete in one alignment.", "database_source": "/path/to/manifest.dm6.json" }