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"982_8KXMVK_4|u1", "983_8KXMVK_4|u1", "985_8KXMVK_4|u1", "986_8KXMVK_4|u1", "987_8KXMVK_4|u1", "988_8KXMVK_4|u1", "989_8KXMVK_4|u1", "98_8KXMVK_4|u1", "990_8KXMVK_4|u1", "991_8KXMVK_4|u1", "992_8KXMVK_4|u1", "993_8KXMVK_4|u1", "994_8KXMVK_4|u1", "995_8KXMVK_4|u1", "996_8KXMVK_4|u1", "997_8KXMVK_4|u1", "998_8KXMVK_4|u1", "999_8KXMVK_4|u1", "99_8KXMVK_4|u1" ], "qualifying_reads": 0, "qualifying_fraction": 0.0, "qualifying_read_ids": [], "candidate_read_species": {}, "qualifying_read_loci": {}, "status": "NOT_DETECTED", "candidates": { "s_cerevisiae": { "label": "Saccharomyces cerevisiae", "role": "spikein_candidate", "reads": 0, "fraction": 0.0, "loci": 0, "status": "NOT_DETECTED", "fraction_ci95": [ 1.0842021724855044e-19, 0.0014832795295675614 ] }, "s_pombe": { "label": "Schizosaccharomyces pombe", "role": "spikein_candidate", "reads": 0, "fraction": 0.0, "loci": 0, "status": "NOT_DETECTED", "fraction_ci95": [ 1.0842021724855044e-19, 0.0014832795295675614 ] }, "lambda": { "label": "Lambda phage", "role": "sequencing_control", "reads": 0, "fraction": 0.0, "loci": 0, "status": "NOT_DETECTED", "fraction_ci95": [ 1.0842021724855044e-19, 0.0014832795295675614 ] }, "phix": { "label": "PhiX174", "role": "sequencing_control", "reads": 0, "fraction": 0.0, "loci": 0, "status": "NOT_DETECTED", "fraction_ci95": [ 1.0842021724855044e-19, 0.0014832795295675614 ] }, "e_coli": { "label": "Escherichia coli", "role": "spikein_candidate", "reads": 0, "fraction": 0.0, "loci": 0, "status": "NOT_DETECTED", "fraction_ci95": [ 1.0842021724855044e-19, 0.0014832795295675614 ] }, "d_melanogaster": { "label": "Drosophila melanogaster", "role": "spikein_candidate", "reads": 0, "fraction": 0.0, "loci": 0, "status": "NOT_DETECTED", "fraction_ci95": [ 1.0842021724855044e-19, 0.0014832795295675614 ] } }, "assignment_counts": { "ambiguous_or_unassigned": 605, "host": 1981 }, "fraction_ci95": [ 1.0842021724855044e-19, 0.0014832795295675614 ], "zero_hit_upper95": 0.0011577717523688635, "command": [ "/path/to/bowtie2", "--very-sensitive-local", "-p", "4", "-x", "/path/to/index", "-U", "/path/to/HL240.inserts.fastq.gz" ], "database_source": "/path/to/manifest.json", "database_manifest": "/path/to/d2aba8b035c32cdecb131b1809d757479200c65ee888a8dc001abde9636a6b59.json", "parameters": { "mapq": 20, "minimum_aligned_bp": 80, "minimum_query_coverage": 0.8, "minimum_identity": 0.85, "minimum_score_margin": 10 }, "interpretation": "Candidate foreign-sequence signal, not proof that spike-in was intentionally added. Experimental spike-in intent must be established from metadata; positive signals require investigation. Zero hits do not establish absence, and the binomial bound assumes independent inserts and perfect detectability. Host and candidate genomes plus Mollicutes/bacterial decoys compete in one alignment." }